2021 AMI Online Salon

Glucagon Binding in Two Steps

Project Details

  • Entrant Name:  Ryan Nini
  • Client: Isabel Romero Calvo & Evelyn Maizels
  • Copyright: Ryan Nini, 2021
  • Medium/software used: VMD, Blender, Adobe Illustrator
  • Final presentation format: Digital Poster
  • Primary Audience: Scientific Audience

Project Description

Glucagon is an important regulatory peptide hormone that controls blood glucose homeostasis during fasting. The structure of its receptor, the Glucagon Receptor (GCGR), is well characterized. Additionally, the two-step binding process for ligands of GCGR and other class-b G-Protein Coupled Receptors is well described. However, the spatial relationship of interacting moieties between GCGR and glucagon in this two-step process is not well visualized. This poster seeks to address this visualization problem in three stages: the natant, inactive form; the initial binding of glucagon to GCGR’s extracellular domain; and the subsequent binding of glucagon to GCGR’s J-domain.